Объем | 100 мкл |
Синонимы | Constitutive NOS, EC-NOS, ECNOS, NOS3, NOSIII |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P29474 |
Иммуноген | Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, IHC, IF,IHC:1:50-1:100, IF:1:100-1:200 |
Примечание | <p>Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. <br /><br />Fulton, D. et al. (1999) Nature 399, 597-601. <br />Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591. <br />Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser1177 |
Области исследований | Neuroscience,Cancer,Cardiovascular,Cell biology,Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS(Phospho-Ser1177) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Phospho-Ser1177) Antibody.
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