Phospho-PKM (Ser37) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | PKM, PK3, OIP3, PK2 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P14618 | | Иммуноген | Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 | | Примечание | <p>Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.</p><p>Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji, , ",ERK1/2-dependent phosphorylation and nuclear ,translocation of PKM2 promotes the Warburg effect.", Nature Cell Biology(2012)|doi:10.1038/ncb2629<br />Received16 August 2012 Accepted24 October 2012 Published online25 November 2012</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser37 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody.
 | Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody.
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Наименование: Phospho-PKM (Ser37) Antibody.