Объем | 50 мкл |
Синонимы | Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1 |
Тип антител | Recombinant Antibody |
Species | Human |
UniProt ID | P37840 |
Иммуноген | A synthesized peptide derived from human Phospho-SNCA (S129) |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:50-1:200, IF:1:20-1:200 |
Клональность | Monoclonal |
Изотип | Rabbit IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Affinity-chromatography |
Области исследований | Neuroscience |
Аббревиатура | Alpha-synuclein |
Примечание | May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. |
Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-RA021912A129phHU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with CSB-RA021912A129phHU at 1:206,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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