| Объем | 100 мкл |
| Синонимы | C-SRC, SRC1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P12931 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme, phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. <br /><br />Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 <br />Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040 <br />Xu W., (1997).Nature 385:595-602 <br />Benes C.H., (2005) Cell 121:271-280</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr529 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src(Phospho-Tyr529) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Phospho-Tyr529) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using Src(Phospho-Tyr529) Antibody.
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Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody.
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Наименование: Phospho-SRC (Tyr529) Antibody.