| Объем | 100 мкл |
| Синонимы | APRF, HIES |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P40763 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from Human STAT3. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. <br /><br />Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. <br />Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804. <br />Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115. <br />Garcia R, et al. (1998) J Biomed Sci. 5: 79-85. <br />Bromberg J F, et al. (1999) Cell. 98: 295-303.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser727 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cardiovascular,Developmental biology,Signal transduction,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela and HepG2 cells untreated or treated with PMA usingSTAT3(Phospho-Ser727) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3(Phospho-Ser727) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using STAT3(Phospho-Ser727) Antibody.
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Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT3 (Phospho-Ser727) Antibody.
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Наименование: Phospho-STAT3 (Ser727) Antibody.