| Объем | 100 мкг |
| Синонимы | Serine/threonine-protein kinase pim-3 (EC 2.7.11.1), PIM3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q86V86 |
| Иммуноген | Recombinant Human Serine/threonine-protein kinase pim-3 protein (278-326AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Serine/threonine-protein kinase pim-3 |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse stomach tissue
All lanes: PIM3 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA771444LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA771444LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA771444LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: PIM3 Antibody.