Объем | 50 мкл |
Синонимы | Phosphomannomutase 2 (PMM 2) (EC 5.4.2.8), PMM2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O15305 |
Иммуноген | Recombinant Human Phosphomannomutase 2 protein (1-246AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Phosphomannomutase 2 |
Области исследований | Cancer, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: PMM2 antibody at 3.8µg/ml
Lane 1: A549 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29, 14 kDa
Observed band size: 29 kDa
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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA018238ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA018238ESR2HU at dilution of 1:100
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Immunoprecipitating PMM2 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA018238ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA018238ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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