Объем | 100 мкл |
Синонимы | replication protein A2, 32kDa |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P15927 |
Иммуноген | Full length fusion protein |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC,ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:30-1:150 |
Примечание | Replication protein A 32 kDa subunit is a protein that in humans is encoded by theRPA2 gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen affinity purification |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA249582(RPA2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ?200)
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Gel: 12%SDS-PAGE,Lysate: 40 ?g,Lane 1-5: 231 cells, Jurkat cells, A549 cells, HUVEC cells, Hela cells,Primary antibody: CSB-PA249582(RPA2 Antibody) at dilution 1/600 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 10 seconds
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