Объем | 50 мкл |
Синонимы | Replication protein A 30 kDa subunit (RP-A p30) (Replication factor A protein 4) (RF-A protein 4), RPA4 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q13156 |
Иммуноген | Recombinant Human Replication protein A 30 kDa subunit protein (1-230AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 |
Примечание | As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: RPA4 antibody at 3µg/ml
Lane 1: K562 whole cell lysate
Lane 2: Mouse spleen tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA622640ESR2HU at dilution of 1:100
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