| Объем | 100 мкг |
| Синонимы | Exopolyphosphatase PRUNE1 (EC 3.6.1.1) (Drosophila-related expressed sequence 17) (DRES-17) (DRES17) (HTcD37) (Protein prune homolog 1) (hPrune), PRUNE1, PRUNE |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q86TP1 |
| Иммуноген | Recombinant Human Protein prune homolog protein (1-168AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA771430LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
All lanes: PRUNE antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 43, 31, 27, 25, 19 kDa
Observed band size: 60 kDa
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IHC image of CSB-PA771430LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-PA771430LA01HU at 1:135, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating PRUNE in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA771430LA01HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA771430LA01HU (8µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)
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Наименование: PRUNE Antibody.