PTK2B (Ab-579) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CADTK, CAK beta, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, EC 2.7.10.2 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q14289 | | Иммуноген | Synthesized non-phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 579 (E-D-Y(p)-Y-K). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption, this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation, this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC, this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN, this requires both SRC and PTK2/PYK2.<br/><br/>Lev S., Nature 376:737-745(1995).<br/>Herzog H., Genomics 32:484-486(1996).<br/>Sasaki H., J. Biol. Chem. 270:21206-21219(1995).<br/> <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 3T3 cells, using PYK2 (Ab-579) antibody.
 | Western blot analysis of extracts from 3T3 cells (Lane 2), using PYK2 (Ab-579) antiobdy. The lane on the left is treated with synthesized peptide.
 | | | |
Информация для заказа |
Наименование: PTK2B (Ab-579) Antibody.