| Объем | 50 мкг |
| Синонимы | POU domain, class 5, transcription factor 1 (Octamer-binding protein 3) (Oct-3) (Octamer-binding protein 4) (Oct-4) (Octamer-binding transcription factor 3) (OTF-3), POU5F1, OCT3 OCT4 OTF3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q01860 |
| Иммуноген | Recombinant Human POU domain, class 5, transcription factor 1 protein (1-360AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| Примечание | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Developmental biology, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: POU domain, class 5, transcription factor 1 antibody at 2µg/ml
Lane 1: jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39, 31 kDa
Observed band size: 39 kDa
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Western Blot
Positive WB detected in: HEK293 whole cell lysate, Mouse brain tissue
All lanes: Pou5f1 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 31 kDa
Observed band size: 39 kDa
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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA11579A0Rb at dilution of 1:100
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IHC image of CSB-PA11579A0Rb diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of NIH/3T3 cells with CSB-PA11579A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: POU5F1 Antibody.