RB1 (Ab-795) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | P105-RB, PP105, PP110, RB-1, RB1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P06400 | | Иммуноген | Peptide sequence around aa.793~797 (P-S-S-P-L) derived from Human Rb. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.<br/><br/>Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.<br/>Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.<br/>Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.<br/>Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from MOLT cells using Rb(Ab-795) Antibody and the same antibody preincubated with blocking peptide.
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Наименование: RB1 (Ab-795) Antibody.