PRKDC AntibodyСпецификация| Объем | 100 мкл | | Синонимы | DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P78527 | | Иммуноген | Synthesized peptide derived from human DNA-PK. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2, negatively regulates CRY1 phosphorylation.<br/><br/>Wesley D. Block, Nucleic Acids Res., Apr 2004, 32: 1967 - 1972.<br/>Katherine S. Pawelczak, Nucleic Acids Res., Jan 2005, 33: 152 - 161.<br/>Eun-Jung Park, Nucleic Acids Res., Dec 2003, 31: 6819 - 6827.<br/>Suisheng Zhang, Nucleic Acids Res., Jan 2004, 32: 1 - 10.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DNA-PK antibody.
 | Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DNA-PK antibody.
 | Western blot analysis of extracts from HeLa cells using DNA-PK antibody.
 | Western blot analysis of extracts from HeLa cells (Lane 2), using DNA-PK antiobdy. The lane on the left is treated with systhesized peptide.
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Наименование: PRKDC Antibody.