Объем | 50 мкг |
Синонимы | E3 ubiquitin-protein ligase RAD18 (EC 2.3.2.27) (Postreplication repair protein RAD18) (hHR18) (hRAD18) (RING finger protein 73) (RING-type E3 ubiquitin transferase RAD18), RAD18, RNF73 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9NS91 |
Иммуноген | Recombinant Human E3 ubiquitin-protein ligase RAD18 protein (1-495AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on Lys-164. Has ssDNA binding activity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: E3 ubiquitin-protein ligase RAD18 antibody at 2µg/ml + COLO205 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using PA889102LA01HU at dilution of 1:100
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