| Объем | 100 мкг |
| Синонимы | Prostasin (EC 3.4.21.-) (Channel-activating protease 1) (CAP1) (Serine protease 8) [Cleaved into: Prostasin light chain, Prostasin heavy chain], PRSS8 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q16651 |
| Иммуноген | Recombinant Human Prostasin protein (185-319AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200 |
| Примечание | Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Prostasin |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, A2780 whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate
All lanes: PRSS8 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 31 kDa
Observed band size: 70 kDa
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Immunofluorescence staining of MCF-7 cells with CSB-PA623091LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: PRSS8 Antibody.