| Объем | 50 мкл |
| Синонимы | 26S proteasome non-ATPase regulatory subunit 6 (26S proteasome regulatory subunit RPN7) (26S proteasome regulatory subunit S10) (Breast cancer-associated protein SGA-113M) (Phosphonoformate immuno-associated protein 4) (Proteasome regulatory particle subunit p44S10) (p42A), PSMD6, KIAA0107 PFAAP4 |
| Клональность | Polyclonal |
| Организм | Human |
| uniprot | Q15008 |
| Иммуноген | Recombinant Human 26S proteasome non-ATPase regulatory subunit 6 protein (355-372AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | 26S proteasome non-ATPase regulatory subunit 6 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate
All lanes: PSMD6 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 42, 52 kDa
Observed band size: 46 kDa
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IHC image of CSB-PA614875PA01HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of MCF-7 cells with CSB-PA614875PA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: PSMD6 Antibody.