| Объем | 100 мкл |
| Синонимы | E3 ubiquitin-protein ligase RNF5 (EC 2.3.2.27) (Protein G16) (RING finger protein 5) (RING-type E3 ubiquitin transferase RNF5) (Ram1 homolog) (HsRma1), RNF5, G16 NG2 RMA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q99942 |
| Иммуноген | Recombinant Human E3 ubiquitin-protein ligase RNF5 protein (1-120AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD, the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation, the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | E3 ubiquitin-protein ligase RNF5 |
| Области исследований | Cancer, Cell biology, Immunology, Microbiology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: RNF5 antibody at 3.08µg/ml
Lane 1: A431 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA857879DSR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA857879DSR2HU at dilution of 1:100
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Наименование: RNF5 Antibody.