Объем | 50 мкг |
Синонимы | Prostaglandin E2 receptor EP4 subtype (PGE receptor EP4 subtype) (PGE2 receptor EP4 subtype) (Prostanoid EP4 receptor), PTGER4, PTGER2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P35408 |
Иммуноген | Recombinant Human Prostaglandin E2 receptor EP4 subtype protein (354-472AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
Примечание | Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Prostaglandin E2 receptor EP4 subtype |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat liver tissue, Rat kidney tissue
All lanes: PTGER4 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA018973LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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