PXN (Ab-31) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | PAXI, PXN, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P49023 | | Иммуноген | Peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | <p>An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions <br /><br />Davidson D, et al. (2001) EMBO J, 20(13): 3414-3426 <br />Kook S, et al. (2000) Mol Biol Cell, 11(3): 929-939 <br />Fleming I, et al. (1999) Proc Natl Acad Sci U S A, 96(3): 1123-1128 <br />Goldberg MB, et al. (2001) Microbiol Mol Biol Rev, 65(4): 595-626 <br />Thomas JT, et al. (1999) Proc Natl Acad Sci U S A, 96(15): 8449-8454</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 cells using Paxillin(Ab-31) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin(Ab-31) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: PXN (Ab-31) Antibody.