SAE1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | AOS1, HSPC140, SAE1, SUA1, SUMO-1 activating enzyme E1 N subunit | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9UBE0 | | Иммуноген | Synthesized peptide derived from Internal of human AOS1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IF,WB:1:500-1:3000, IF:1:100-1:500 | | Примечание | The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.<br/><br/>Yoshiaki Azuma, FASEB J, Jun 2001, 10.1096.<br/>Walter Makous, Invest. Ophthalmol. Vis. Sci., Sep 2006, 47: 4160 - 4167. <br/>Hsin-Pei Shih, J. Cell Sci., Mar 2002, 115: 1259. <br/>Thomas Buschmann, J. Biol. Chem., Oct 2001, 276: 40389 - 40395.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Immunofluorescence analysis of HuvEc cells, using AOS1 antibody.
 | Western blot analysis of extracts from 293 cells, using AOS1 antibody.
 | Western blot analysis of extracts from Colo cells, HepG2 cells and 293 cells using AOS1 antibody. The lane on the left is treated with synthesized peptide.
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Наименование: SAE1 Antibody.