Объем | 100 мкг |
Синонимы | Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91), SPHK1, SPHK SPK |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9NYA1 |
Иммуноген | Recombinant Human Sphingosine kinase 1 protein (1-384AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Sphingosine kinase 1 |
Области исследований | Cancer, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse skeletal muscle tissue
All lanes: SPHK1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 52, 44 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA022564LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA022564LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA022564LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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