| Объем | 100 мкл |
| Синонимы | Histone-lysine N-methyltransferase SETDB1 (EC 2.1.1.43) (ERG-associated protein with SET domain) (ESET) (Histone H3-K9 methyltransferase 4) (H3-K9-HMTase 4) (Lysine N-methyltransferase 1E) (SET domain bifurcated 1), SETDB1, KIAA0067 KMT1E |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q15047 |
| Иммуноген | Recombinant Human Histone-lysine N-methyltransferase SETDB1 protein (1-397AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 |
| Примечание | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SETDB1 antibody at 5µg/ml
Lane 1: Rat adrenal gland tissue
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 144, 45 kDa
Observed band size: 144 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA619960ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA619960ESR1HU at dilution of 1:100
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Наименование: SETDB1 Antibody.