RPA1 AntibodyСпецификация| Объем | 100 мкг | | Синонимы | Replication protein A 70 kDa DNA-binding subunit (RP-A p70) (Replication factor A protein 1) (RF-A protein 1) (Single-stranded DNA-binding protein) [Cleaved into: Replication protein A 70 kDa DNA-binding subunit, N-terminally processed], RPA1, REPA1 RPA70 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P27694 | | Иммуноген | Recombinant Human Replication protein A 70 kDa DNA-binding subunit protein (2-616AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:3000, IHC:1:20-1:200 | | Примечание | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Replication protein A 70 kDa DNA-binding subunit | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA020088LA01HU at dilution of 1:100
 | Western Blot
Positive WB detected in: A549 whole cell lysate, Rat lung tissue, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue
All lanes: RPA1 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
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Информация для заказа |
Наименование: RPA1 Antibody.