| Объем | 50 мкг |
| Синонимы | Protein shisa-5 (Putative NF-kappa-B-activating protein 120) (Scotin), SHISA5, SCOTIN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q8N114 |
| Иммуноген | Recombinant Human Protein shisa-5 protein (56-137AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, IP, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500, IP:1:200-1:2000 |
| Примечание | Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA818677LA01HU at dilution of 1:100
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Immunofluorescence staining of A549 cells with CSB-PA818677LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating SHISA5 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA818677LA01HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA818677LA01HU (6µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)
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Наименование: SHISA5 Antibody.