| Объем | 100 мкг |
| Синонимы | E3 ubiquitin-protein ligase RNF126 (EC 2.3.2.27) (RING finger protein 126), RNF126 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9BV68 |
| Иммуноген | Recombinant Human E3 ubiquitin-protein ligase RNF126 protein (39-225AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed:24981174). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | E3 ubiquitin-protein ligase RNF126 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: RNF126 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 34 kDa
Observed band size: 36 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA019828LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA019828LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA019828LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: RNF126 Antibody.