| Объем | 50 мкл |
| Синонимы | Protein salvador homolog 1 (45 kDa WW domain protein) (hWW45), SAV1, WW45 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H4B6 |
| Иммуноген | Recombinant Human Protein salvador homolog 1 protein (1-210AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Protein salvador homolog 1 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA875672ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA875672ESR2HU at dilution of 1:100
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Immunofluorescent analysis of A549 cells using CSB-PA875672ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: 293T whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: SAV1 antibody at 1.62µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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Immunoprecipitating SAV1 in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA875672ESR2HU in NIH/3T3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA875672ESR2HU (5µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)
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Наименование: SAV1 Antibody.