| Объем | 50 мкл |
| Синонимы | Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3), SMAD3, MADH3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P84022 |
| Иммуноген | Recombinant Human Mothers against decapentaplegic homolog 3 protein (1-230AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Metabolism, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SMAD3 antibody at 8µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: A431 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49, 44, 36, 26 kDa
Observed band size: 49 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA021788ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA021788ESR2HU at dilution of 1:100
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Immunoprecipitating SMAD3 in Jurkat whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA021788ESR2HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA021788ESR2HU (4µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)
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IHC image of CSB-PA021788ESR2HU diluted at 1:388 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of A549 cells with CSB-PA021788ESR2HU at 1:129, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: SMAD3 Antibody.