| Объем | 50 мкг |
| Синонимы | Superoxide dismutase [Mn], mitochondrial (EC 1.15.1.1), SOD2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04179 |
| Иммуноген | Recombinant Human Superoxide dismutase [Mn], mitochondrial protein (64-175AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:600, IF:1:200-1:500, IP:1:200-1:2000 |
| Примечание | Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Cancer, Cardiovascular, Cell biology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SOD2 antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Mouse liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25, 21, 19, 20 kDa
Observed band size: 25 kDa
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Western blot
All lanes: SOD2 antibody at 4 µg/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Mouse heart tissue
Lane 3: Mouse liver tissue
Lane 4: Mouse lung tissue
Lane 5: Mouse kidney tissue
Lane 6: Mouse brain tissue
Lane 7: Mouse skeletal muscle tissue
Lane 8: Mouse thymus tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25, 21, 19, 20 kDa
Observed band size: 25 kDa
 |
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA022398LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat heart tissue, Mouse brain tissu
All lanes: SOD2 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25, 21, 19, 20 kDa
Observed band size: 25 kDa
 |
IHC image of CSB-PA022398LA01HU diluted at 1:560 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA022398LA01HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating SOD2 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA022398LA01HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA022398LA01HU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
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Наименование: SOD2 Antibody.