Объем | 100 мкг |
Синонимы | Tubulin beta-3 chain (Tubulin beta-4 chain) (Tubulin beta-III), TUBB3, TUBB4 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q13509 |
Иммуноген | Recombinant Human Tubulin beta-3 chain protein (1-210AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: TUBB3 antibody at 1.5µg/ml
Lane 1: Mouse brain tissue
Lane 2: A549 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: A431 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 51, 43 kDa
Observed band size: 51 kDa
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Immunohistochemistry of paraffin-embedded colocretal cancer using CSB-PA03879A0Rb at dilution of 1:50
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Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: TUBB3 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 43 kDa
Observed band size: 51 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA03879A0Rb at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA03879A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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