SMN1 AntibodyСпецификация| Объем | 100 мкг | | Синонимы | Survival motor neuron protein (Component of gems 1) (Gemin-1), SMN1, SMN2, SMN SMNT, SMNC | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q16637 | | Иммуноген | Recombinant Human Survival motor neuron protein (3-194AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 | | Примечание | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Survival motor neuron protein | | Области исследований | Epigenetics and Nuclear Signaling, Neuroscience, Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate
All lanes: SMN1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32, 29, 31, 28 kDa
Observed band size: 40, 60 kDa
 | Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA614982LA01HU at dilution of 1:100
 | Immunofluorescent analysis of HepG2 cells using CSB-PA614982LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: SMN1 Antibody.