| Объем | 50 мкг |
| Синонимы | Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.4.19.12) (Deubiquitinating enzyme 5) (Isopeptidase T) (Ubiquitin thioesterase 5) (Ubiquitin-specific-processing protease 5), USP5, ISOT |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P45974 |
| Иммуноген | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 5 protein (201-500AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: USP5 antibody at 10µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse brain tissue
Lane 3: A549 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: A375 whole cell lysate
Lane 6: U251 whole cell lysate
Lane 7: HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 96, 94 kDa
Observed band size: 96 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA025742LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA025742LA01HU at dilution of 1:100
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Наименование: USP5 Antibody.