Объем | 50 мкг |
Синонимы | Protein sprouty homolog 1 (Spry-1), SPRY1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O43609 |
Иммуноген | Recombinant Human Protein sprouty homolog 1 protein (1-178AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:200-1:500 |
Примечание | May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022621LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, Mouse liver tissue
All lanes: SPRY1 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunofluorescence staining of PC3 cells with CSB-PA022621LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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