| Объем | 50 мкг |
| Синонимы | Sequestosome-1 (EBI3-associated protein of 60 kDa) (EBIAP) (p60) (Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa) (Ubiquitin-binding protein p62), SQSTM1, ORCA OSIL |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13501 |
| Иммуноген | Recombinant Human Sequestosome-1 protein (16-310AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, IP, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500, IP:1:200-1:2000 |
| Примечание | Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Sequestosome-1 |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA615696LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA615696LA01HU at dilution of 1:100
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Immunofluorescent analysis of MCF-7 cells using CSB-PA615696LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating SQSTM1 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA615696LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA615696LA01HU (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: SQSTM1 Antibody.