Объем | 100 мкг |
Синонимы | Transducin-like enhancer protein 4 (Grg-4) (Groucho-related protein 4), TLE4, GRG4 KIAA1261 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q04727 |
Иммуноген | Recombinant Human Transducin-like enhancer protein 4 protein (284-394AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IF, Recommended dilution: IF:1:50-1:200 |
Примечание | Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Transducin-like enhancer protein 4 |
Области исследований | Epigenetics and Nuclear Signaling, Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of Hela cells with CSB-PA523988LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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