STAT1 (Ab-727) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | ISGF-3, STAT91, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P42224 | | Иммуноген | Peptide sequence around aa.725~729 (P-M-S-P-E) derived from Human STAT1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. <br/><br/>Yuan ZM, et al. (1999) Nature.399 (6738): 814-817.<br/>Schindler C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A 89:7836-7839.<br/>Strausberg R.L (2002). Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903.<br/>Quelle F.W., (1995) J. Biol. Chem. 270:20775-20780.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from MCF7 cells using STAT1(Ab-727) Antibody and the same antibody preincubated with blocking peptide.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1(Ab-727) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: STAT1 (Ab-727) Antibody.