| Объем | 50 мкл |
| Синонимы | Vacuolar protein sorting-associated protein 4A (EC 3.6.4.6) (Protein SKD2) (VPS4-1) (hVPS4), VPS4A, VPS4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UN37 |
| Иммуноген | Recombinant Human Vacuolar protein sorting-associated protein 4A protein (1-120AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Vacuolar protein sorting-associated protein 4A |
| Области исследований | Cell biology, Microbiology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: VPS4Aantibody at 4.28µg/ml
Lane 1: Mouse kidney tissue
Lane 2: Rat gonadal tissue
Lane 3: Mouse brain tissue
Lane 4: U87 whole cell lysate
Lane 5: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA891974DSR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA891974DSR2HU at dilution of 1:100
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Наименование: VPS4A Antibody.