Объем | 50 мкг |
Синонимы | Protein sprouty homolog 4 (Spry-4), SPRY4 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9C004 |
Иммуноген | Recombinant Human Protein sprouty homolog 4 protein (1-299AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IF, Recommended dilution: IF:1:200-1:500 |
Примечание | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of PC3 cells with CSB-PA866335LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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