| Объем | 50 мкг |
| Синонимы | Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF), Intracellular domain 1 (ICD1), Intracellular domain 2 (ICD2), C-domain 1, C-domain 2, Tumor necrosis factor, soluble form], TNF, TNFA TNFSF2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P01375 |
| Иммуноген | Recombinant Human Tumor necrosis factor protein (77-233AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Cardiovascular, Immunology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: TNF antibody at 2µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue, Mouse liver tissue
All lanes: TNF antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA07427A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA07427A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA07427A0Rb at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA07427A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: TNF Antibody.