YY1AP1 AntibodyСпецификация| Объем | 50 мкг | | Синонимы | YY1-associated protein 1, Hepatocellular carcinoma susceptibility protein1, YY1AP1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9H869 | | Иммуноген | Recombinant Human YY1-associated protein 1 protein (335-466AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200 | | Примечание | Associates with the INO80 chromatin remodeling complex, which is responsible for transcriptional regulation, DNA repair, and replication (PubMed:27939641). Enhances transcription activation by YY1 (PubMed:14744866). Plays a role in cell cycle regulation (PubMed:17541814, PubMed:27939641). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | YY1-associated protein 1 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: YY1AP1 antibody at 4.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDa
Observed band size: 88, 51 kDa
 | IHC image of CSB-PA884488LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of HepG2 cells with CSB-PA884488LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 | Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysate
All lanes: YY1AP1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDa
Observed band size: 88 kDa
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Наименование: YY1AP1 Antibody.