ZAP70 (Ab-319) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P43403 | | Иммуноген | Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | <p>ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions <br /><br />Salomon AR, et al. (2003) Proc Natl Acad Sci U S A, 100(2): 443-448 <br />Ku GM, et al. (2001) EMBO J, 20(3): 457-465 <br />Tang J, et al. (1999) Proc Natl Acad Sci U S A, 96(17): 9775-9780 <br />Zhao Q, et al. (1996) Mol Cell Biol, 16(12): 6765-6774 <br />Williams BL, et al. (1999) EMBO J, 18(7): 1832-1844 <br />Adjali O, et al. (2005) J Clin Invest, 115(8): 2287-2295.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Tags & Cell Markers,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HL60 cells using Zap-70(Ab-319) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70(Ab-319) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: ZAP70 (Ab-319) Antibody.