| Объем | 50 мкг |
| Синонимы | Troponin T, cardiac muscle (TnTc) (Cardiac muscle troponin T) (cTnT), TNNT2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P45379 |
| Иммуноген | Recombinant Human Troponin T, cardiac muscle protein (138-298AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Troponin T, cardiac muscle protein |
| Области исследований | Cardiovascular, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Troponin T, cardiac muscle antibody at 2µg/ml
Lane 1: Mouse heart tissue
Lane 2: Rat heart tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36, 32, 35, 34, 31 kDa
Observed band size: 36 kDa
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Western Blot
Positive WB detected in: Mouse skeletal muscle
All lanes: TNNT2 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 32, 35, 34, 31 kDa
Observed band size: 32, 36 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA10047A0Rb at dilution of 1:20
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IHC image of CSB-PA10047A0Rb diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA10047A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: TNNT2 Antibody.