Объем | 100 мкл |
Синонимы | Threonine--tRNA ligase, cytoplasmic (EC 6.1.1.3) (Threonyl-tRNA synthetase) (ThrRS), TARS |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P26639 |
Иммуноген | Recombinant Human Threonine--tRNA ligase, cytoplasmic protein (1-230AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | actin cytoskeleton, cytoplasm, cytosol, extracellular exosome, protein homodimerization activity, RNA binding, threonine-tRNA ligase activity, threonyl-tRNA aminoacylation, translation, tRNA aminoacylation for protein translation |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Threonine--tRNA ligase, cytoplasmic |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: TARS antibody at 4.85µg/ml
Lane 1: A549 whole cell lysate
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 84, 87 kDa
Observed band size: 84 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA023131ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA023131ESR2HU at dilution of 1:100
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Immunoprecipitating TARS in A549 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA023131ESR2HU in A549 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA023131ESR2HU (8µg) + A549 whole cell lysate (500µg)
Lane 3: A549 whole cell lysate (10µg)
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