| Объем | 100 мкг |
| Синонимы | Tubulin alpha-4A chain (Alpha-tubulin 1) (Testis-specific alpha-tubulin) (Tubulin H2-alpha) (Tubulin alpha-1 chain), TUBA4A, TUBA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P68366 |
| Иммуноген | Recombinant Human Tubulin alpha-4A chain protein (20-183AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Tubulin alpha-4A chain |
| Области исследований | Isotype/Loading Controls, Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: TUBA4A antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 49 kDa
Observed band size: 50, 24 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA09499A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA09499A0Rb at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA09499A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating TUBA4A in K562 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA09499A0Rb in K562 whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA09499A0Rb (4µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
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Наименование: TUBA4A Antibody.