| Объем | 50 мкг |
| Синонимы | Troponin I, cardiac muscle (Cardiac troponin I), TNNI3, TNNC1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P19429 |
| Иммуноген | Recombinant Human Troponin I, cardiac muscle protein (2-210AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, IP, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cardiovascular, Developmental biology, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA07937A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA07937A0Rb at dilution of 1:100
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Immunoprecipitating TNNI3 in Rat heart tissue
Lane 1: Rabbit control IgG instead of CSB-PA07937A0Rb in Rat heart tissue.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA07937A0Rb (8µg) + Rat heart tissue (500µg)
Lane 3: Rat heart tissue (20µg)
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Immunofluorescence staining of Hela cells with CSB-PA07937A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: TNNI3 Antibody.