| Объем | 50 мкг |
| Синонимы | Thiopurine S-methyltransferase (EC 2.1.1.67) (Thiopurine methyltransferase), TPMT |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P51580 |
| Иммуноген | Recombinant Human Thiopurine S-methyltransferase protein (4-244AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | TPMT is an enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Thiopurine S-methyltransferase protein |
| Области исследований | Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Thiopurine S-methyltransferase antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00585A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00585A0Rb at dilution of 1:100
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Immunoprecipitating TPMT in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA00585A0Rb in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA00585A0Rb (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
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Наименование: TPMT Antibody.