VDAC1 AntibodyСпецификацияОбъем | 100 мкл | Синонимы | Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC | Тип антител | Recombinant Antibody | Species | Human | UniProt ID | P21796 | Иммуноген | A synthesized peptide derived from human VDAC1 | Видовая специфичность | Human, Mouse, Rat | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200 | Клональность | Monoclonal | Изотип | Rabbit IgG | Коньюгат | Non-conjugated | Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Affinity-chromatography | Области исследований | Tags & Cell Markers | Аббревиатура | Voltage-dependent anion-selective channel protein 1 | Примечание | Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules, in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, LO2 whole cell lysate, Raji whole cell lysate, Rat heart tissue, Mouse brain tissue
All lanes: VDAC1 antibody at 0.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 KDa
Observed band size: 31 KDa
| IHC image of CSB-RA025821A0HU diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
| IHC image of CSB-RA025821A0HU diluted at 1:73.125 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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