| Объем | 50 мкг |
| Синонимы | Bone morphogenetic protein 4 (BMP-4) (Bone morphogenetic protein 2B) (BMP-2B), BMP4, BMP2B DVR4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P12644 |
| Иммуноген | Recombinant Human Bone morphogenetic protein 4 protein (303-408AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat, Zebrafish |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000 |
| Примечание | Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Bone morphogenetic protein 4 antibody at 10µg/ml
Lane 1: Rat liver tissue
Lane 2: A549 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
 |
Western blot
All lanes: BMP4 antibody at 10µg/ml
Lane 1: Mouse kidney tissue
Lane 2: Mouse liver tissue
Lane 3: Rat liver tissue
Lane 4: Rat kidney tissue
Lane 5: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 23, 47, 59 kDa
 |
Western blot
All lanes: BMP-4 antibody at 6 µg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
 |
Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA05799A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA05799A0Rb at dilution of 1:100
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Western Blot
Positive WB detected in: Mouse brain tissue, Mouse skeletal muscle tissue, Zebrafish
All lanes: Bmp4 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
 |
IHC image of CSB-PA05799A0Rb diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA05799A0Rb diluted at 1:600 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: BMP4 Antibody.