Объем | 50 мкг |
Синонимы | Serine/threonine-protein kinase WNK4 (EC 2.7.11.1) (Protein kinase lysine-deficient 4) (Protein kinase with no lysine 4), WNK4, PRKWNK4 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q96J92 |
Иммуноген | Recombinant Human Serine/threonine-protein kinase WNK4 protein (828-1106AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Serine/threonine-protein kinase WNK4 |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA842690LA01HU at dilution of 1:100
|
Immunofluorescent analysis of HepG2 cells using CSB-PA842690LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|
Immunofluorescent analysis of PC-3 cells using CSB-PA842690LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|
| |