| Объем | 100 мкг |
| Синонимы | Ubiquitin carboxyl-terminal hydrolase 14 (EC 3.4.19.12) (Deubiquitinating enzyme 14) (Ubiquitin thioesterase 14) (Ubiquitin-specific-processing protease 14), USP14, TGT |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P54578 |
| Иммуноген | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 14 protein (1-494AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Ubiquitin carboxyl-terminal hydrolase 14 antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57, 53, 55 kDa
Observed band size: 57 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA025704LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA025704LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA025704LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: USP14 Antibody.